ITPKC Polyklonaler Antikörper

ITPKC Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14267-1-PBS

Synonyme

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  WB analysis using 14267-1-AP (same clone as 14267-1-PBS)

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 14267-1-AP (ITPKC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 14267-1-AP (same clone as 14267-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 14267-1-AP (ITPKC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 14267-1-AP (same clone as 14267-1-PBS)

  IP result of anti-ITPKC (IP:14267-1-AP, 4ug; Detection:14267-1-AP 1:400) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14267-1-AP (ITPKC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14267-1-AP (ITPKC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14267-1-AP (same clone as 14267-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using ITPKC antibody 14267-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14267-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ITPKC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ITPKC fusion protein Ag5583
• Vollständiger Name: inositol 1,4,5-trisphosphate 3-kinase C
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 100-105 kDa
• GenBank-Zugangsnummer: BC060788
• Gene symbol: ITPKC
• Gene ID (NCBI): 80271
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITPKC(Inositol-trisphosphate 3-kinase C) is also named as IP3KC and belongs to the inositol phosphokinase (IPK) family. It can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate and is relatively weakly activated by the calcium-calmodulin complex. ITPKC also acts as a negative regulator of T-cell activation through the Ca(2+)/NFAT signaling pathway, and the C allele may contribute to immune hyperreactivity in Kawasaki disease(PMID:17160344).