ITPR3 Polyklonaler Antikörper

ITPR3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 20729-1-AP

Synonyme

InsP3R3, IP3R, IP3R 3, IP3R3

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  WB analysis using 20729-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20729-1-AP (ITPR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse small intestine using 20729-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20729-1-AP (ITPR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 20729-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20729-1-AP (ITPR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 20729-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20729-1-AP (ITPR3 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, Maus-Cerebellum-Gewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

20729-1-AP bindet in WB, IHC, ELISA ITPR3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: inositol 1,4,5-triphosphate receptor, type 3
• Berechnetes Molekulargewicht: 304 kDa
• Beobachtetes Molekulargewicht: 250-300 kDa
• GenBank-Zugangsnummer: NM_002224
• Gene symbol: ITPR3
• Gene ID (NCBI): 3710
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITPRs family have three members: ITPR1, ITPR2 and ITPR3. For ITPR3, it is associated with cervical squamous cell carcinoma, glioblastoma, cholangiocarcinoma and other cancers. Inositol 1,4, 5-triphosphate receptor type 3 (ITPR3) was found to be apical-located in bile duct cells, and disruption of lipid raft in the segregated bile duct unit (IBDU) redistributes ITPR3 and impairs Ca2+ waves. (PMID: 35580861, PMID:35852334)

Publikationen

Species	Application	Title
mouse	WB	J Ethnopharmacol; Zhizichi decoction alleviates depressive-like behaviors through modulating mitochondria-associated membrane via the IP3R3-GRP75-VDAC1 complex; Authors - Ye Liu
	WB	Adv Sci (Weinh); Massage-Mimicking Nanosheets Mechanically Reorganize Inter-organelle Contacts to Restore Mitochondrial Functions in Parkinson's Disease; Authors - Tianqi Li
human	WB	Sci Rep; Polydatin exerts therapeutic effects on myelodysplastic syndrome by inhibiting the protein expression of oncogenes via hypermethylation in vitro; Authors - Qingbing Zhou
human	WB	Mol Cell Endocrinol; High glucose elevates intracellular calcium level and induces ferroptosis in glomerular endothelial cells through the miR-223-3p/ITPR3 pathway; Authors - Dekai Wang