ICAM-1/CD54 Polyklonaler Antikörper

ICAM-1/CD54 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30324-1-AP

Synonyme

Icam-1, CD54, MALA-2, Icam1, ICAM 1

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  WB analysis using 30324-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30324-1-AP (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30324-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 30324-1-AP (Icam-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 30324-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30324-1-AP (Icam-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 30324-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30324-1-AP (Icam-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 30324-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30324-1-AP (Icam-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 30324-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Icam-1 antibody (30324-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mauslebergewebe, Mausmilzgewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30324-1-AP bindet in WB, IHC, IF-P, ELISA ICAM-1/CD54 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: intercellular adhesion molecule 1
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 95-110 kDa
• GenBank-Zugangsnummer: NM_010493
• Gene symbol: ICAM-1
• Gene ID (NCBI): 15894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ICAM-1 (Intercellular Adhesion Molecule-1, CD54) is a glycosylated, transmembrane protein, a member of Ig super family. This protein involves in cell adhesion by interacting with a number of 2 integrin family members such as LFA-1 and Mac-1. Its expression is regulated by chemokines. This antibody is raised against mouse Icam-1.