Iduronate 2 sulfatase Monoklonaler Antikörper

Iduronate 2 sulfatase Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A3F9

Kat-Nr. : 66112-1-PBS

Synonyme

IDS, 1A3F9, Alpha-L-iduronate sulfate sulfatase, EC:3.1.6.13, Iduronate 2-sulfatase

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  WB analysis of human placenta using 66112-1-Ig (same clone as 66112-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66112-1-Ig (same clone as 66112-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66112-1-Ig (same clone as 66112-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66112-1-Ig (same clone as 66112-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66112-1-Ig (same clone as 66112-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66112-1-Ig (same clone as 66112-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66112-1-Ig (same clone as 66112-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66112-1-Ig (same clone as 66112-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Iduronate 2 sulfatase antibody (66112-1-Ig, Clone: 1A3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66112-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Iduronate 2 sulfatase und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Iduronate 2 sulfatase fusion protein Ag19095
• Vollständiger Name: iduronate 2-sulfatase
• Berechnetes Molekulargewicht: 312aa,35 kDa; 550aa,62 kDa
• Beobachtetes Molekulargewicht: 76 kDa, 55 kDa
• GenBank-Zugangsnummer: BC006170
• Gene symbol: IDS
• Gene ID (NCBI): 3423
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).