IgM Isotype Control Monoklonaler Antikörper

IgM Isotype Control Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgM, lambda

Getestete Reaktivität

n/a

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

MOPC-104E

Kat-Nr. : 65243-1-Ig

Synonyme

IgM

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  FC experiment of human whole blood using 65243-1-Ig

  Human whole blood was treated with ACK lysis buffer then 1x10^6 cells were surface stained with 0.2 ug Mouse IgM Isotype Control (MOPC-104E) (65243-1-Ig) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) (red), or CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) alone (black dashed), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human peripheral blood platelets using 65243-1-Ig

  100ul human platelet-rich plasma (diluted 1:2 in PBS) was surface stained with 0.2 ug Mouse IgM Isotype Control (65243-1-Ig, Clone: MOPC-104E) (red) or Anti-Human CD36 (65248-1-Ig) (green) or unstained (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

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Geprüfte Anwendungen

Erfolgreiche Detektion in FC

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65243-1-Ig bindet in FC IgM Isotype Control und zeigt Reaktivität mit n/a

• Getestete Reaktivität: n/a
• Wirt / Isotyp: Maus / IgM, lambda
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: IgM Isotype Control
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

The MOPC-104E immunoglobulin is useful as an isotype-matched control for mouse IgM antibodies. B1355S dextran, nigerosyl-alpha (1-3)-nigerose, and nigerosyl-alpha (1-3)-glucose have abilities to bind to MOPC 104E immunoglobulin (PMID: 6165777). This antibody has been quality-tested for flow cytometry as negative control.