IkB Alpha Rekombinanter Antikörper

IkB Alpha Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5C24

Kat-Nr. : 80019-1-PBS

Synonyme

IKBA, IκB α, NFKBIA, 5C24, I kappa B alpha

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  WB analysis using 80019-1-RR (same clone as 80019-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80019-1-RR (IkB alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 80019-1-RR (same clone as 80019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80019-1-RR (IkB Alpha antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 80019-1-RR (same clone as 80019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80019-1-RR (IkB Alpha antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 80019-1-RR (same clone as 80019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 80019-1-RR (same clone as 80019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 80019-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80019-1-RR against Human IkB Alpha were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

80019-1-PBS bindet in WB, IHC, Indirect ELISA IkB Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IkB Alpha fusion protein Ag0345
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa and 35 kDa
• GenBank-Zugangsnummer: BC002601
• Gene symbol: IkB Alpha
• Gene ID (NCBI): 4792
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. IkB alpha can be observed as a band at 36 kDa in human cell lysates, and 35 kDa in mouse and rat cell ysates.