IL-1 beta Polyklonaler Antikörper

IL-1 beta Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 29530-1-AP

Synonyme

IL 1 beta, IL-1 beta, Il1b, interleukin 1 beta, Interleukin-1 beta

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  WB analysis using 29530-1-AP

  Untreated RAW 264.7 cells, RAW 264.7 cell (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for at least 3h) were subjected to SDS PAGE followed by western blot with 29530-1-AP (Il1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit LPS und Brefeldin A behandelte RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

29530-1-AP bindet in WB, ELISA IL-1 beta und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: interleukin 1 beta
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: NM_031512
• Gene symbol: IL-1 beta
• Gene ID (NCBI): 24494
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.

Publikationen

Species	Application	Title
mouse	WB	Ann Med; Astragaloside IV ameliorate acute alcohol-induced liver injury in mice via modulating gut microbiota and regulating NLRP3/caspase-1 signaling pathway; Authors - Shan Wu
mouse	WB	Front Pharmacol; Dapagliflozin promotes angiogenesis in hindlimb ischemia mice by inducing M2 macrophage polarization; Authors - Heng Yang
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Farnesoid X receptor overexpression prevents hepatic steatosis through inhibiting AIM2 inflammasome activation in nonalcoholic fatty liver disease; Authors - Shuai Xu
mouse	WB	iScience; The mechanisms and therapeutic potential of clopidogrel in mitigating diabetic cardiomyopathy in db/db mice; Authors - Bing Li
rat	WB	Clin Nutr; Untargeted metabolomics and mendelian randomization analysis identify alpha-linolenic acid and linoleic acid as novel biomarkers of perioperative neurocognitive dysfunction; Authors - Xinxin Yang
human	WB	Int Immunopharmacol; Adipose-derived stem cell exosomes loaded with icariin attenuated M1 polarization of macrophages via inhibiting the TLR4/Myd88/NF-κB signaling pathway; Authors - Qiqi Yan