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IL-10 Rekombinanter Antikörper

IL-10 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240430A11

Kat-Nr. : 82191-5-PBS

Synonyme

Il10, IL-10,IL10, CSIF, Il 10, interleukin 10

Filter:
  • Sandwich ELISA standard curve of MP00507-4

    Sandwich ELISA standard curve of MP00507-4

    Sandwich ELISA standard curve of MP00507-4, IL-10 Recombinant Matched Antibody Pair - PBS only. 82191-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0026.82191-7-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

  • Sample test of MP00507-4

    Sample test of MP00507-4

    Mouse splenocytes (1 x 10^cells/mL) were cultured for 4 days in RPMI supplemented with 10% fetal calf serum, 100 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of LPS. The mean IL-10 concentration was determined to be 7.59 pg/mL in unstimulated spleen cell culture supernatant, 2,461.0 pg/mL in IFN-γ and LPS stimulated spleen cell culture supernatant. Mouse splenocytes (1 x 10^cells/mL) were cultured for 3 days in RPMI supplemented with 10% fetal calf serum,1 μg/mL of LPS. The mean IL-10 concentration was determined to be 11.30 pg/mL in unstimulated spleen cell culture supernatant, 143.99 pg/mL in LPS stimulated spleen cell culture supernatant.

Geprüfte Anwendungen

Produktinformation

82191-5-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test IL-10 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen IL-10 fusion protein Eg0026
Vollständiger Name interleukin 10
Berechnetes Molekulargewicht 21 kDa
GenBank-ZugangsnummerNM_010548
Gene symbol Il10
Gene ID (NCBI) 16153
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.