IL-15RA Rekombinanter Antikörper

IL-15RA Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242618H6

Kat-Nr. : 85671-2-PBS

Synonyme

CD215, IL-15 receptor subunit alpha, Il15ra, IL-15R-alpha, Interleukin-15 receptor subunit alpha

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  WB analysis using 85671-2-RR (same clone as 85671-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85671-2-RR (Il15ra antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85671-2-PBS in a different storage buffer formulation.

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  WB analysis using 85671-2-RR (same clone as 85671-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85671-2-RR (Il15ra antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85671-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02016-3

  Sandwich ELISA standard curve of MP02016-3, Mouse IL-15RA Recombinant Matched Antibody Pair - PBS only. 85671-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2687. 85671-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP02016-3

  The mean IL-15RA concentration was determined to be 1,332.06 ng/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load.

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Geprüfte Anwendungen

Produktinformation

85671-2-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test IL-15RA und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-15RA fusion protein Eg2687
• Vollständiger Name: interleukin 15 receptor, alpha chain
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_008358.2
• Gene symbol: Il15ra
• Gene ID (NCBI): 16169
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights of 55-65 kDa have been reported by some researches (PMID: 10480910; 15976182; 15671076; 17912462).