IL3RA/CD123 Polyklonaler Antikörper

IL3RA/CD123 Polyklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30012-1-AP

Synonyme

Il3ra, Sut-1, IL-3R-alpha, IL-3RA, IL-3R subunit alpha

---

• 

  WB analysis using 30012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30012-1-AP (Il3ra antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30012-1-AP (Il3ra antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse brain using 30012-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Il3ra antibody (30012-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30012-1-AP bindet in WB, IF-P, ELISA IL3RA/CD123 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL3RA/CD123 fusion protein Ag32002
• Vollständiger Name: interleukin 3 receptor, alpha chain
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa, 60-70 kDa
• GenBank-Zugangsnummer: NM_008369
• Gene symbol: Il3ra
• Gene ID (NCBI): 16188
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-3RA, also named as CD123 and SUT-1, is the alpha subunit of the interleukin 3 (IL-3) receptor, which is part of the type I cytokine receptor superfamily (PMID: 25043189). It can regulate the proliferation, survival, and differentiation of hematopoietic cells. In mouse, there are two classes of high-affinity IL-3 receptors. One contains an IL-3-specific beta subunit and the other contains the beta subunit also shared by high-affinity IL-5 and GM-CSF receptors. This antibody is specific for mouse Il3ra.