CD126/IL-6R alpha Rekombinanter Antikörper

CD126/IL-6R alpha Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230371E7

Kat-Nr. : 83209-2-PBS

Synonyme

CD126, Il6ra, IL 6R, Il6r, IL-6R alpha

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  Sandwich ELISA sample test of MP00182-2

  J774A.1 mouse reticulum cell sarcoma macrophage cells (1 x 106 cells/mL) were cultured for 3 days in DMEM containing 10% fetal bovine serum. An aliquot of the cell culture supernatants were removed, assayed for mouse IL-6R alpha, and measured 402.5 pg/mL. Liver tissue from one mouse was homogenized and seeded into 100 mL of RPMI containing 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate, and 50 μM β-mercaptoethanol for 3 days. An aliquot of the cell culture supernate was removed, assayed for mouse IL-6R alpha, and measured 345.1 pg/mL.

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  Sandwich ELISA sample test of MP00182-2

  Serum of sixteen mice were measured. The IL-6R alpha concentration of detected samples was determined to be 10,180.3 pg/mL with a range of 6,065.2-15,362.2 pg/mL.

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  Sandwich ELISA standard curve of MP00182-2

  Sandwich ELISA standard curve of MP00182-2, Mouse CD126/IL-6R alpha Monoclonal Matched Antibody Pair - PBS only. 98024-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0664. 83209-2-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

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Geprüfte Anwendungen

Produktinformation

83209-2-PBS bindet in Sandwich ELISA, Indirect ELISA CD126/IL-6R alpha und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD126/IL-6R alpha fusion protein Eg0664
• Vollständiger Name: interleukin 6 receptor, alpha
• Berechnetes Molekulargewicht: 50KD
• GenBank-Zugangsnummer: NM_010559.3
• Gene symbol: Il6ra
• Gene ID (NCBI): 16194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.