Ins1 Rekombinanter Antikörper

Ins1 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

240798G4

Kat-Nr. : 83867-1-RR

Synonyme

Insulin, insulin1, insulin I, Ins2 rs1, Ins-1

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  WB analysis of rat pancreas using 83867-1-RR

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 83867-1-RR (insulin1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 83867-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 83867-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 83867-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse pancreas using 83867-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Ins1 antibody (83867-1-RR, Clone: 240798G4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 83867-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using insulin1 antibody (83867-1-RR, Clone: 240798G4, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).SST-Specific antibody (84351-1-RR, Clone: 240816D9,red ) at dilution of 1:400 and FlexAble CoraLite® Plus 594 Antibody Labeling Kit for Rabbit IgG(KFA009), Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse pancreas using 83867-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Ins1 antibody (83867-1-RR, Clone: 240798G4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 83867-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Ins1 antibody (83867-1-RR, Clone: 240798G4 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83867-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83867-1-RR against Mouse Ins1 were performed. The affinity constant is 0.21 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Pankreasgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:5000-1:20000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83867-1-RR bindet in WB, IHC, IF-P, ELISA Ins1 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Ins1 fusion protein Ag29988
• Vollständiger Name: insulin I
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: NM_008386
• Gene symbol: Ins1
• Gene ID (NCBI): 16333
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Insulin 1 is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.