Integrin Alpha 3 Monoklonaler Antikörper

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Integrin Alpha 3 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3F9G4

Kat-Nr. : 66070-1-PBS

Synonyme

CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a

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  WB analysis of A549 using 66070-1-Ig (same clone as 66070-1-PBS)

  WB result of Integrin Alpha 3 antibody (66070-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 3 transfected A549 cells. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 66070-1-Ig (same clone as 66070-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (ITGA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66070-1-Ig (same clone as 66070-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66070-1-Ig (same clone as 66070-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66070-1-Ig (same clone as 66070-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66070-1-Ig (same clone as 66070-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66070-1-Ig (same clone as 66070-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66070-1-Ig (same clone as 66070-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66070-1-PBS bindet in WB, IHC, Indirect ELISA Integrin Alpha 3 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Integrin Alpha 3 fusion protein Ag17293
• Vollständiger Name: integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
• Berechnetes Molekulargewicht: 1066 aa, 119 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC150190
• Gene symbol: Integrin alpha 3
• Gene ID (NCBI): 3675
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.