Integrin Alpha V Rekombinanter Antikörper

Integrin Alpha V Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242376D3

Kat-Nr. : 84883-5-RR

Synonyme

ITGAV, 242376D3, CD51, Integrin alpha-V, Integrin alpha-V heavy chain

---

• 

  WB analysis using 84883-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocellular cancer using 84883-5-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocellular cancer using 84883-5-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 84883-5-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 84883-5-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 84883-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Integrin Alpha V antibody (84883-5-RR, Clone: 242376D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 84883-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84883-5-RR against Human Integrin Alpha V were performed. The affinity constant is 0.121 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, humanes Plazenta-Gewebe, HUVEC-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: human hepatocellular cancer, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84883-5-RR bindet in WB, IHC, IF/ICC, ELISA Integrin Alpha V und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Integrin Alpha V fusion protein Ag25825
• Vollständiger Name: integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• Berechnetes Molekulargewicht: 1048 aa, 116 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC136442
• Gene symbol: Integrin alpha V
• Gene ID (NCBI): 3685
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha V, also known as CD51, is a type I transmembrane glycoprotein composed of an extracellular domain, a transmembrane region and cytoplasmic domain (PMID: 2430295; 1690718). It can form heterodimers with one of the five beta integrin subunits (beta 1, 3, 5, 6, 8) that recognize ligands containing the sequence Arg-Gly-Asp, such as vitronectin, fibronectin, and osteopontin (PMID: 37087799). Integrin alpha V mainly plays a role in extracellular matrix-mediated cell adhesion and migration, differentiation, wound healing, inflammation, tumorigenesis, proliferation, angiogenesis, and metastasis.