Integrin Beta 1/CD29 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Integrin Beta 1/CD29 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12594-1-PBS

Synonyme

ITGB1, CD29, FNRB, Glycoprotein IIa, GPIIA

---

• 

  WB analysis using 12594-1-AP (same clone as 12594-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12594-1-AP (same clone as 12594-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 12594-1-AP (same clone as 12594-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 1 antibody (12594-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12594-1-AP (same clone as 12594-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Integrin Beta 1 antibody (12594-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

12594-1-PBS bindet in WB, IHC, IF/ICC, ELISA Integrin Beta 1/CD29 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin Beta 1/CD29 fusion protein Ag3078
• Vollständiger Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 100-130 kDa
• GenBank-Zugangsnummer: BC020057
• Gene symbol: Integrin beta 1
• Gene ID (NCBI): 3688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrin beta-1 (ITGB1), also named as CD29, is a single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.