Integrin Beta 4 Monoklonaler Antikörper

Integrin Beta 4 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C2A11

Kat-Nr. : 66922-2-PBS

Synonyme

ITGB4, 1C2A11, CD104, GP150, Integrin beta-4

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  WB analysis using 66922-2-Ig (same clone as 66922-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

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  WB analysis of SW 1990 using 66922-2-Ig (same clone as 66922-2-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 66922-2-Ig (same clone as 66922-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 66922-2-Ig (same clone as 66922-2-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66922-2-Ig (same clone as 66922-2-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin beta-4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66922-2-Ig (same clone as 66922-2-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66922-2-Ig (same clone as 66922-2-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66922-2-Ig (same clone as 66922-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 4 antibody (66922-2-Ig, Clone: 1C2A11 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66922-2-PBS bindet in WB, IHC, IF/ICC, ELISA Integrin Beta 4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Integrin Beta 4 fusion protein Ag17395
• Vollständiger Name: integrin, beta 4
• Berechnetes Molekulargewicht: 1752 aa, 195 kDa
• Beobachtetes Molekulargewicht: 200-210 kDa
• GenBank-Zugangsnummer: BC126411
• Gene symbol: Integrin beta 4
• Gene ID (NCBI): 3691
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.