Integrin alpha 1 Polyklonaler Antikörper

Integrin alpha 1 Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 29042-1-AP

Synonyme

ITGA1, CD49 antigen-like family member A, CD49a, integrin, alpha 1, Laminin and collagen receptor

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  WB analysis using 29042-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 29042-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Integrin alpha-1 antibody (29042-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 29042-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using Integrin alpha-1 antibody (29042-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IF-Fro: Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

29042-1-AP bindet in WB, IHC, IF-P, IF-Fro, ELISA Integrin alpha 1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin alpha 1 fusion protein Ag30613
• Vollständiger Name: integrin, alpha 1
• Berechnetes Molekulargewicht: 1179 aa, 131 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC137121
• Gene symbol: Integrin alpha 1
• Gene ID (NCBI): 3672
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

Publikationen

Species	Application	Title
human	WB	Development; TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration; Authors - Binbin Li
human	WB	PeerJ; Production of recombinant human epidermal growth factor fused with HaloTag protein and characterisation of its biological functions; Authors - Mengru Bai