Integrin alpha 1 Monoklonaler Antikörper

Integrin alpha 1 Monoklonal Antikörper für WB, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C4F2

Kat-Nr. : 68211-1-PBS

Synonyme

ITGA1, Laminin and collagen receptor, integrin, alpha 1, CD49a, CD49 antigen-like family member A

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  WB analysis of HepG2 using 68211-1-Ig (same clone as 68211-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68211-1-Ig (Integrin alpha-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68211-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 68211-1-Ig (same clone as 68211-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68211-1-Ig (Integrin alpha-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68211-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 68211-1-Ig (same clone as 68211-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Integrin alpha 1 antibody (68211-1-Ig, Clone: 1C4F2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68211-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 68211-1-Ig (same clone as 68211-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Integrin alpha 1 antibody (68211-1-Ig, Clone: 1C4F2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68211-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50423-3

  Cytometric bead array standard curve of MP50423-3, Integrin alpha-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68211-4-PBS. Detection antibody: 68211-1-PBS. Standard:Ag30631. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68211-1-PBS bindet in WB, IF-P, Cytometric bead array, Indirect ELISA Integrin alpha 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Integrin alpha 1 fusion protein Ag30631
• Vollständiger Name: integrin, alpha 1
• Berechnetes Molekulargewicht: 1179 aa, 131 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC137121
• Gene symbol: Integrin alpha 1
• Gene ID (NCBI): 3672
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).