Integrin alpha 2 Polyklonaler Antikörper

Integrin alpha 2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30703-1-AP

Synonyme

ITGA2, BR, CD49B, Collagen receptor, GPIa

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  WB analysis using 30703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30703-1-AP (Integrin alpha 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30703-1-AP (Integrin alpha-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 30703-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ITGA2 antibody (30703-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, A431-Zellen, HeLa-Zellen, MDA-MB-231-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30703-1-AP bindet in WB, IF/ICC, ELISA Integrin alpha 2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin alpha 2 fusion protein Ag32771
• Vollständiger Name: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• Berechnetes Molekulargewicht: 126 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: NM_002203
• Gene symbol: Integrin alpha 2
• Gene ID (NCBI): 3673
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-2 (ITGA2, CD49b) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor (VLA-2) for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin.