Integrin alpha-5/CD49e Monoklonaler Antikörper

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• KD/KO Validated

Integrin alpha-5/CD49e Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3B9E5

Kat-Nr. : 68561-1-Ig

Synonyme

Integrin Alpha-5, ITGA5, 3B9E5, CD49 antigen-like family member E, CD49e

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  WB analysis using 68561-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 68561-1-Ig

  WB result of Integrin alpha-5/CD49e antibody (68561-1-Ig; 1:8000; room temperature for 1.5 hours) with wild-type and Integrin alpha-5/CD49e knockout HAP1 cells.

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  WB analysis using 68561-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 68561-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maus-Kolongewebe, Maus-Dünndarmgewebe, Hausschwein-Kolongewebe, Ratten-Kolongewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

68561-1-Ig bindet in WB, ELISA Integrin alpha-5/CD49e und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Integrin alpha-5/CD49e fusion protein Ag33642
• Vollständiger Name: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Berechnetes Molekulargewicht: 115 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC008786
• Gene symbol: Integrin alpha 5
• Gene ID (NCBI): 3678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

Publikationen

Species	Application	Title
human	WB	Genomics; The noncausal association between a loss-of-function CLCN2 variant and childhood absence epilepsy; Authors - Wei Li