Integrin alpha-5/CD49e Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Integrin alpha-5/CD49e Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241685A3

Kat-Nr. : 84468-4-RR

Synonyme

Integrin Alpha-5, ITGA5, 241685A3, CD49 antigen-like family member E, CD49e

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  WB analysis using 84468-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84468-4-RR (Integrin alpha-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 84468-4-RR

  WB result of Integrin alpha-5/CD49e antibody (84468-4-RR; 1:4000; room temperature for 1.5 hours) with wild-type and sg-Integrin alpha-5/CD49e transfected HAP1 cells.

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  IF Staining of HeLa using 84468-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Integrin alpha-5 antibody (84468-4-RR, Clone: 241685A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84468-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84468-4-RR against Human Integrin alpha-5 were performed. The affinity constant is 1.24 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84468-4-RR bindet in WB, IF/ICC, ELISA Integrin alpha-5/CD49e und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Berechnetes Molekulargewicht: 115 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC008786
• Gene symbol: Integrin alpha 5
• Gene ID (NCBI): 3678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).