Integrin alpha-5/CD49e Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Integrin alpha-5/CD49e Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241684E8

Kat-Nr. : 84468-5-RR

Synonyme

Integrin Alpha-5, ITGA5, 241684E8, CD49 antigen-like family member E, CD49e

---

• 

  WB analysis of HAP1 using 84468-5-RR

  WB result of Integrin alpha-5/CD49e antibody (84468-5-RR; 1:4000; room temperature for 1.5 hours) with wild-type and sg-Integrin alpha-5/CD49e transfected HAP1 cells.

• 

  WB analysis using 84468-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84468-5-RR (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84468-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84468-5-RR against Human Integrin alpha-5 were performed. The affinity constant is 1.41 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84468-5-RR bindet in WB, ELISA Integrin alpha-5/CD49e und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Berechnetes Molekulargewicht: 115 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC008786
• Gene symbol: Integrin alpha 5
• Gene ID (NCBI): 3678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).