Integrin alpha-5/CD49e Rekombinanter Antikörper

Integrin alpha-5/CD49e Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241685D4

Kat-Nr. : CL488-98171

Synonyme

Integrin Alpha-5, ITGA5, 241685D4, CD49 antigen-like family member E, CD49e

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  FC experiment of human PBMCs using CL488-98171

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD3, and 5 ul CoraLite® Plus 488 Anti-Human Integrin alpha-5/CD49e Rabbit RecAb (CL488-98171, Clone: 241685D4) or CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL488-98171

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human Integrin alpha-5/CD49e Rabbit RecAb (CL488-98171, Clone: 241685D4) (red) or CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-98171 bindet in FC Integrin alpha-5/CD49e und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Berechnetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC008786
• Gene symbol: Integrin alpha 5
• Gene ID (NCBI): 3678
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 491 nm / 516 nm
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha-5 (ITGA5, also known as CD49e), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).