Integrin alpha-8 Polyklonaler Antikörper

Integrin alpha-8 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30714-1-PBS

Synonyme

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  WB analysis using 30714-1-AP (same clone as 30714-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 30714-1-AP (same clone as 30714-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 30714-1-AP (same clone as 30714-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse lung using 30714-1-AP (same clone as 30714-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using Integrin alpha-8 antibody (30714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse lung using 30714-1-AP (same clone as 30714-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using Integrin alpha-8 antibody (30714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30714-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Integrin alpha-8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin alpha-8 fusion protein Ag33507
• Vollständiger Name: integrin, alpha 8
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: NM_003638
• Gene symbol: Integrin alpha 8
• Gene ID (NCBI): 8516
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. Integrin alpha-8 is a single-pass type I membrane protein that forms a heterodimer with integrin beta-1 to serve as a receptor for RGD-containing matrix molecules (fibronectin, vitronectin, tenascin C, osteopontin, and nephronectin) (PMID: 33000355). Integrin alpha-8 beta-1 plays a critical role in epithelial-mesenchymal interactions and functions in the genesis of kidney and probably of other organs (PMID: 9054500; 19769957). The apparent molecular weight of integrin alpha-8 is larger than the calculated molecular weight of 117 kDa, possibly due to glycosylation (PMID: 7768999).