Integrin Beta 1/CD29 Rekombinanter Antikörper

Integrin Beta 1/CD29 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1K20

Kat-Nr. : 82729-3-PBS

Synonyme

ITGB1, 1K20, CD29, FNRB, Glycoprotein IIa

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  WB analysis using 82729-3-RR (same clone as 82729-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82729-3-RR (Integrin beta-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82729-3-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 82729-3-RR (same clone as 82729-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82729-3-RR (Integrin beta-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82729-3-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 82729-3-RR (same clone as 82729-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82729-3-RR (Integrin beta-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82729-3-PBS in a different storage buffer formulation.

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  IF Staining of Customer Sample using 82729-3-RR (same clone as 82729-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded Customer Sample customer using Integrin Beta 1 antibody (82729-3-RR, Clone: 1K20 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82729-3-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82729-3-PBS bindet in WB, IHC, IF-P, Indirect ELISA Integrin Beta 1/CD29 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Integrin Beta 1/CD29 fusion protein Ag3078
• Vollständiger Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 110-140 kDa
• GenBank-Zugangsnummer: BC020057
• Gene symbol: Integrin beta 1
• Gene ID (NCBI): 3688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrin beta-1 (ITGB1), also named as CD29, is a single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.