Integrin beta-5 Rekombinanter Antikörper

Integrin beta-5 Rekombinant Antikörper für WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240401G11

Kat-Nr. : 83514-3-PBS

Synonyme

ITGB5, 240401G11, Integrin beta 5, integrin, beta 5

---

• 

  WB analysis using 83514-3-RR (same clone as 83514-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP00512-4

  The mean Integrin Beta 5 concentration was determined to be 275.71 pg/mL in Hela cell extract based on a 2.8 mg/mL extract load and 190.06 pg/mL in MCF-7 cell extract based on a 3.1 mg/mL extract load.

• 

  Sandwich ELISA standard curve of MP00512-4

  Sandwich ELISA standard curve of MP00512-4, Integrin Beta 5 Recombinant Matched Antibody Pair - PBS only. 83514-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag29783.83514-1-PBS was HRP conjugated as the detection antibody. Range: 9.766-625 pg/mL.

• 

  IHC staining of human ovary cancer using 83514-3-RR (same clone as 83514-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 83514-3-RR (same clone as 83514-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83514-3-RR (same clone as 83514-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Integrin beta-5 Recombinant antibody (83514-3-RR, Clone:240401G11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83514-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83514-3-PBS against Human Integrin beta-5 was performed. The affinity constant is 2.00 nM.

• 

  Affinity and Kinetic Characterization of 83514-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83514-3-RR against Human Integrin beta-5 were performed. The affinity constant is 0.955 nM.

---

Geprüfte Anwendungen

Produktinformation

83514-3-PBS bindet in WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA Integrin beta-5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Integrin beta-5 fusion protein Ag29783
• Vollständiger Name: integrin, beta 5
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 88-100 kDa
• GenBank-Zugangsnummer: BC006541
• Gene symbol: Integrin beta 5
• Gene ID (NCBI): 3693
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITGB5, also known as Integrin beta-5. It is expected to be located in the plasma membrane and mitochondria, which is i ubiquitinated in placenta and gall bladder. ITGB5 is a member of integrins and was proved to be associated with pathological processes in several tumors (PMID: 34966851). It is reported that TGB5 is highly expressed in hepatocellular carcinoma (HCC), and miR-185 regulates the expression of β- catenin through the ITGB5-dependent manner and affects the proliferation and migration of HCC cells (PMID: 35223869). The molecular weight of ITGB5 is 88 kDa, and the glycosylation modification also exists.