Integrin beta-8 Polyklonaler Antikörper

Integrin beta-8 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 29775-1-AP

Synonyme

ITGB8, integrin, beta 8, Integrin beta 8

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  WB analysis using 29775-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29775-1-AP (Integrin beta-8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 29775-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29775-1-AP (Integrin beta-8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 29775-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29775-1-AP (Integrin beta-8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

29775-1-AP bindet in WB, IHC, ELISA Integrin beta-8 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin beta-8 fusion protein Ag31137
• Vollständiger Name: integrin, beta 8
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: NM_002214
• Gene symbol: Integrin beta 8
• Gene ID (NCBI): 3696
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-8 (ITGB8), belongs to the integrin beta chain family, contains an N-terminal signal peptide, a large extracellular domain that includes four cysteine-rich repeats, a transmembrane domain, and a C-terminal cytoplasmic domain (PMID: 1918072). Integrin beta-8 associates with integrin alpha-V (ITGAV) to form an αvβ8 heterodimer which has been shown to bind vitronectin, fibronectin, and a latency-associated peptide of TGF-β1/3 (PMID: 27033701). Integrin beta-8 contains multiple potential N-linked glycosylation sites. The observed molecular mass of approximately 95 kDa for integrin beta-8 is larger than the predicted 86 kDa, consistent with substantial glycosylation (PMID: 1918072).

Publikationen

Species	Application	Title
	IHC	Sci Adv; AKR1B10 dictates c-Myc stability to suppress colorectal cancer metastasis via PP2A nitration; Authors - Xiaoxue Wu
human	IHC	Medicine (Baltimore); Prognostic value and potential molecular mechanism of ITGB superfamily members in hepatocellular carcinoma; Authors - Haixiang Xie
		Nat Commun; Dietary nucleic acids promote oral tolerance through innate sensing pathways in mice; Authors - Tao Yang