Integrin beta-8 Polyklonaler Antikörper

Integrin beta-8 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29775-1-PBS

Synonyme

ITGB8, integrin, beta 8, Integrin beta 8

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  WB analysis using 29775-1-AP (same clone as 29775-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29775-1-AP (Integrin beta-8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29775-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 29775-1-AP (same clone as 29775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29775-1-AP (Integrin beta-8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29775-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 29775-1-AP (same clone as 29775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29775-1-AP (Integrin beta-8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29775-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29775-1-PBS bindet in WB, IHC, Indirect ELISA Integrin beta-8 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin beta-8 fusion protein Ag31137
• Vollständiger Name: integrin, beta 8
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: NM_002214
• Gene symbol: Integrin beta 8
• Gene ID (NCBI): 3696
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-8 (ITGB8), belongs to the integrin beta chain family, contains an N-terminal signal peptide, a large extracellular domain that includes four cysteine-rich repeats, a transmembrane domain, and a C-terminal cytoplasmic domain (PMID: 1918072). Integrin beta-8 associates with integrin alpha-V (ITGAV) to form an αvβ8 heterodimer which has been shown to bind vitronectin, fibronectin, and a latency-associated peptide of TGF-β1/3 (PMID: 27033701). Integrin beta-8 contains multiple potential N-linked glycosylation sites. The observed molecular mass of approximately 95 kDa for integrin beta-8 is larger than the predicted 86 kDa, consistent with substantial glycosylation (PMID: 1918072).