Integrin beta-8 Rekombinanter Antikörper

Integrin beta-8 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241549A12

Kat-Nr. : 84558-1-RR

Synonyme

ITGB8, integrin, beta 8, Integrin beta 8, 241549A12

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  WB analysis using 84558-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84558-1-RR (Integrin beta-8 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84558-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84558-1-RR against Human Integrin beta-8 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:9000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84558-1-RR bindet in WB, ELISA Integrin beta-8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Integrin beta-8 fusion protein Ag31161
• Vollständiger Name: integrin, beta 8
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: NM_002214
• Gene symbol: Integrin beta 8
• Gene ID (NCBI): 3696
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-8 (ITGB8), belongs to the integrin beta chain family, contains an N-terminal signal peptide, a large extracellular domain that includes four cysteine-rich repeats, a transmembrane domain, and a C-terminal cytoplasmic domain (PMID: 1918072). Integrin beta-8 associates with integrin alpha-V (ITGAV) to form an αvβ8 heterodimer which has been shown to bind vitronectin, fibronectin, and a latency-associated peptide of TGF-β1/3 (PMID: 27033701). Integrin beta-8 contains multiple potential N-linked glycosylation sites. The observed molecular mass of approximately 95-100 kDa for integrin beta-8 is larger than the predicted 86 kDa, consistent with substantial glycosylation (PMID: 1918072).