IFN-beta Polyklonaler Antikörper

IFN-beta Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

Kat-Nr. : 27506-1-AP

Synonyme

IFN beta, IFNB1, IFN β1, IFNB, IFN-β1

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  WB analysis of Recombinant protein using 27506-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 27506-1-AP (IFN Beta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 27506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IFN-beta antibody (27506-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  IF Staining of L02 using 27506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using IFN-beta antibody (27506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 27506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 27506-1-AP (IFN-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Produktinformation

27506-1-AP bindet in WB, IHC, IF/ICC, ELISA IFN-beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFN-beta fusion protein Ag26326
• Vollständiger Name: IFN beta
• Berechnetes Molekulargewicht: 187 aa, 22 kDa
• GenBank-Zugangsnummer: BC069314
• Gene symbol: IFN beta1
• Gene ID (NCBI): 3456
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interferon-beta (IFN-β) is a type I interferon, which is a group of signaling proteins made and released by host cells in response to the presence of several viruses, including double-stranded RNA produced by many viruses. IFN-β is a single protein species and is molecularly related to IFN-α subtypes, although it is antigenically distinct from them. It is mainly produced by fibroblasts and plays a crucial role in the innate immune response against viruses. IFN-β can inhibit viral replication by interfering with the virus's RNA or DNA, thus suppressing viral growth. It also enhances the cytotoxic activity of natural killer (NK) cells and promotes the expression of major histocompatibility complex (MHC) class I molecules.

Publikationen

Species	Application	Title
human	IHC	J Mater Chem B; Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating; Authors - Bin Yan
human	WB	Int Immunopharmacol; Polysaccharides from Platycodonis Radix ameliorated respiratory syncytial virus-induced epithelial cell apoptosis and inflammation through activation of miR-181a-mediated Hippo and SIRT1 pathways.; Authors - Juan-Juan Li
human	WB	Amino Acids; Escherichia coli infection activates the production of IFN-α and IFN-β via the JAK1/STAT1/2 signaling pathway in lung cells.; Authors - Yan Jin
human	WB	BMC Vet Res; The role of IBV PL1pro in virus replication and suppression of host innate immune responses; Authors - Weirong Liu
human	IF	Cell Death Dis; Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion.; Authors - Tongtian Zhuang
		Virology; Role of heparan sulfate in the Zika virus entry, replication, and cell death.; Authors - Huixin Gao