Involucrin Rekombinanter Antikörper

Involucrin Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240566D8

Kat-Nr. : 83649-5-PBS

Synonyme

IVL, 240566D8

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  WB analysis using 83649-5-RR (same clone as 83649-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83649-5-RR (Involucrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83649-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 83649-5-RR (same clone as 83649-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83649-5-RR (Involucrin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83649-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 83649-5-RR (same clone as 83649-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83649-5-RR (Involucrin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83649-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83649-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83649-5-RR against Human Involucrin were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83649-5-PBS bindet in WB, IHC, ELISA Involucrin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Involucrin fusion protein Ag29585
• Vollständiger Name: involucrin
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: NM_005547
• Gene symbol: Involucrin
• Gene ID (NCBI): 3713
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, but it also forms 120 kDa dimers, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502, 12150517)