Islet 1 Rekombinanter Antikörper

Islet 1 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240833B2

Kat-Nr. : 83784-6-PBS

Synonyme

ISL1, ISLET1, ISL LIM homeobox 1, Isl 1, Insulin gene enhancer protein ISL-1

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  WB analysis using 83784-6-RR (same clone as 83784-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83784-6-RR (Islet 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83784-6-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00771-4

  Sandwich ELISA standard curve of MP00771-4, Human Islet 1 Recombinant Matched Antibody Pair - PBS only. 83784-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33541. 83784-6-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

• 

  Sample test of MP00771-4

  The mean Islet 1 concentration was determined to be 1.05 ng/mL in SH-SY5Y cell extract based on a 3.40 mg/mL extract load.

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Geprüfte Anwendungen

Produktinformation

83784-6-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test Islet 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Islet 1 fusion protein Ag33541
• Vollständiger Name: ISL LIM homeobox 1
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39-45 kDa
• GenBank-Zugangsnummer: BC031213
• Gene symbol: Islet 1
• Gene ID (NCBI): 3670
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts. Ubiquitinated probably by WWP1 E3 ubiquitin ligase; ubiquitination is followed by protein degradation.