JAK1 Monoklonaler Antikörper

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• KD/KO Validated

JAK1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3H7A8

Kat-Nr. : 66466-1-PBS

Synonyme

JAK 1, JAK1, JAK1A, JAK1B, Janus kinase 1, JTK3, Tyrosine protein kinase JAK1

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  WB analysis using 66466-1-Ig (same clone as 66466-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66466-1-Ig (same clone as 66466-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 66466-1-Ig (same clone as 66466-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66466-1-Ig (same clone as 66466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66466-1-Ig (same clone as 66466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66466-1-Ig (same clone as 66466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66466-1-Ig (same clone as 66466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66466-1-PBS bindet in WB, IHC, ELISA JAK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: JAK1 fusion protein Ag17940
• Vollständiger Name: Janus kinase 1 (a protein tyrosine kinase)
• Berechnetes Molekulargewicht: 1154 aa, 133 kDa
• Beobachtetes Molekulargewicht: 133 kDa
• GenBank-Zugangsnummer: BC132729
• Gene symbol: JAK1
• Gene ID (NCBI): 3716
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Janus kinase 1(JAK1), is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 is essential for signaling for certain type I and type II cytokines. JAK1 plays a critical role in initiating responses to multiple major cytokine receptor families. Expression of JAK1 in cancer cells enables individual cells to contract, potentially allowing them to escape their tumor and metastasize to other parts of the body.