JAK2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

JAK2 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

80

Rezensionen (1)

Kat-Nr. : 17670-1-AP

Synonyme

JAK 2, JAK2, Janus kinase 2, JTK10, Tyrosine protein kinase JAK2

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 17670-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17670-1-AP (JAK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 17670-1-AP

  IP Result of anti-JAK2 (IP:17670-1-AP, 4ug; Detection:17670-1-AP 1:300) with Jurkat cells lysate 3800ug.

• 

  IHC staining of human kidney using 17670-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17670-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17670-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17670-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 75 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

17670-1-AP bindet in WB, IP, IHC, IF, ELISA JAK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JAK2 fusion protein Ag11264
• Vollständiger Name: Janus kinase 2 (a protein tyrosine kinase)
• Berechnetes Molekulargewicht: 1132 aa, 130 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC039695
• Gene symbol: JAK2
• Gene ID (NCBI): 3717
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Janus kinases (JAKs) are non-receptor tyrosine kinases that are activated by cytokine binding to cytokine receptors resulting in stimulation of the JAK-STAT signaling pathway. Aberrant activation of this signaling pathway is associated with numerous immune disorders and cancer. This antibody can detect full-length band of 130 kDa, and another bands 150 kDa after posttranslational modification.

Publikationen

Species	Application	Title
mouse	WB	J Neuroinflammation; Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders; Authors - Jingxian Lun
human	WB,IP	Cell Death Dis; 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer; Authors - Yanan Wang
mouse	WB	Theranostics; Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief.; Authors - Jiqian Zhang
human	WB	Arch Med Res; Extracellular Vesicles from Carcinoma-associated Fibroblasts Promote EMT of Salivary Adenoid Cystic Carcinoma Via IL-6; Authors - Hyat Ahmed Ibrahim Al-Raimi
mouse	WB	Oxid Med Cell Longev; Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.; Authors - Wei Wang
rat	IF	Brain Behav Immun; Interleukin-6: Its role and mechanisms in rescuing depression-like behaviors in rat models of depression.; Authors - Peng Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

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