JAK2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

JAK2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(102)

Rezensionen (1)

Kat-Nr. : 17670-1-AP

Synonyme

Tyrosine protein kinase JAK2, JTK10, Janus kinase 2, JAK-2, JAK 2

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  WB analysis of NIH/3T3 using 17670-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17670-1-AP (JAK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 17670-1-AP

  IP result of anti-JAK2 (IP:17670-1-AP, 4ug; Detection:17670-1-AP 1:300) with NIH/3T3 cells lysate 3440 ug.

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  IHC staining of human kidney using 17670-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17670-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17670-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17670-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 93 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

17670-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA JAK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JAK2 fusion protein Ag11264
• Vollständiger Name: Janus kinase 2 (a protein tyrosine kinase)
• Berechnetes Molekulargewicht: 1132 aa, 130 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC039695
• Gene symbol: JAK2
• Gene ID (NCBI): 3717
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Janus kinases (JAKs) are non-receptor tyrosine kinases that are activated by cytokine binding to cytokine receptors resulting in stimulation of the JAK-STAT signaling pathway. Aberrant activation of this signaling pathway is associated with numerous immune disorders and cancer.

Publikationen

Species	Application	Title
rat	WB	Adv Healthc Mater; Dual-Barb Microneedle with JAK/STAT Inhibitor-Loaded Nanovesicles Encapsulation for Tendinopathy; Authors - Minhao Chen
mouse	WB	J Neuroinflammation; Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders; Authors - Jingxian Lun
human	WB,IP	Cell Death Dis; 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer; Authors - Yanan Wang
mouse	WB	Theranostics; Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief.; Authors - Jiqian Zhang
human	WB	Arch Med Res; Extracellular Vesicles from Carcinoma-associated Fibroblasts Promote EMT of Salivary Adenoid Cystic Carcinoma Via IL-6; Authors - Hyat Ahmed Ibrahim Al-Raimi
human	WB	Biomed Pharmacother; Molecular mechanism through which Tripterygium hypoglaucum (Lévl.) Hutch alleviates psoriasis; Authors - Yumei Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other