JAK2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

JAK2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17670-1-PBS

Synonyme

Tyrosine protein kinase JAK2, JTK10, Janus kinase 2, JAK-2, JAK 2

---

• 

  WB analysis of NIH/3T3 using 17670-1-AP (same clone as 17670-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17670-1-AP (JAK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17670-1-PBS in a different storage buffer formulation.

• 

  IP experiment of NIH/3T3 using 17670-1-AP (same clone as 17670-1-PBS)

  IP result of anti-JAK2 (IP:17670-1-AP, 4ug; Detection:17670-1-AP 1:300) with NIH/3T3 cells lysate 3440 ug. This data was developed using the same antibody clone with 17670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17670-1-AP (same clone as 17670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17670-1-AP (same clone as 17670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 17670-1-AP (same clone as 17670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 17670-1-AP (same clone as 17670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17670-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

17670-1-PBS bindet in WB, IHC, IP, ELISA JAK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JAK2 fusion protein Ag11264
• Vollständiger Name: Janus kinase 2 (a protein tyrosine kinase)
• Berechnetes Molekulargewicht: 1132 aa, 130 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC039695
• Gene symbol: JAK2
• Gene ID (NCBI): 3717
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Janus kinases (JAKs) are non-receptor tyrosine kinases that are activated by cytokine binding to cytokine receptors resulting in stimulation of the JAK-STAT signaling pathway. Aberrant activation of this signaling pathway is associated with numerous immune disorders and cancer.