JAKMIP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

JAKMIP1 Polyklonal Antikörper für FC, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13846-1-AP

Synonyme

Gababrbp, JAKMIP1, JAMIP1, Marlin 1, MARLIN1

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Galerie der Validierungsdaten

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  WB analysis of mouse spleen using 13846-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13846-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13846-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13846-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13846-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13846-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 13846-1-AP

  IP Result of anti-JAKMIP1 (IP:13846-1-AP, 3ug; Detection:13846-1-AP 1:1000) with Jurkat cells lysate 4000ug.

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  IHC staining of human gliomas using 13846-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13846-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 13846-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug JAKMIP1 antibody (13846-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, HEK-293-Zellen, Jurkat-Zellen, Maushodengewebe, Mauslebergewebe, Y79-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13846-1-AP bindet in WB, IP, IHC, IF, FC,ELISA JAKMIP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JAKMIP1 fusion protein Ag4816
• Vollständiger Name: janus kinase and microtubule interacting protein 1
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC047075
• Gene symbol: JAKMIP1
• Gene ID (NCBI): 152789
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Neuron; JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.; Authors - Jamee M Berg ; KO Validated
human	WB,IHC	Biomed Pharmacother; Overexpression of JAKMIP1 associates with Wnt/beta-catenin pathway activation and promotes cancer cell proliferation in vitro.; Authors - Okai Isaac I