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JAM-A/CD321 Rekombinanter Antikörper

JAM-A/CD321 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250060H5

Kat-Nr. : 86229-1-PBS

Synonyme

CD321, F11r, Jam1, JAM-1, JAM-A

Filter:
  • Western Blot (WB) analysis of various lysates using JAM-A/CD321 Recombinant antibody (86229-1-RR)

    WB analysis using 86229-1-RR (same clone as 86229-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86229-1-RR (F11r(CD321) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86229-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using JAM-A/CD321 Recombinant antibody (86229-1-RR)

    WB analysis using 86229-1-RR (same clone as 86229-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86229-1-RR (F11r(CD321) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86229-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of bEnd.3 cells using JAM-A/CD321 Recombinant antibody (86229-1-RR)

    IF Staining of bEnd.3 using 86229-1-RR (same clone as 86229-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using JAM-A/CD321 antibody (86229-1-RR, Clone: 250060H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86229-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86229-1-PBS bindet in WB, IF/ICC, Indirect ELISA JAM-A/CD321 und zeigt Reaktivität mit Maus, Ratten

Getestete Reaktivität Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen JAM-A/CD321 fusion protein Eg3117
Vollständiger Name F11 receptor
Berechnetes Molekulargewicht32 kDa
Beobachtetes Molekulargewicht33 kDa
GenBank-ZugangsnummerNM_172647.2
Gene symbol F11r
Gene ID (NCBI) 16456
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Junctional Adhesion Molecule-A (JAM-A), also known as CD321 or F11R, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is primarily located in the tight junctions of epithelial and endothelial cells and is also expressed on circulating platelets and leukocytes (PMID: 34533648). Targeting F11R/JAM-A with monoclonal antibodies or peptide antagonists has shown promise in inhibiting tumor growth and metastasis in mouse models (PMID: 37563645).