JAM-A/CD321 Rekombinanter Antikörper

JAM-A/CD321 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250060H5

Kat-Nr. : 86229-1-RR

Synonyme

CD321, F11r, Jam1, JAM-1, JAM-A

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  WB analysis using 86229-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86229-1-RR (F11r(CD321) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86229-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86229-1-RR (F11r(CD321) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of bEnd.3 using 86229-1-RR

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using JAM-A/CD321 antibody (86229-1-RR, Clone: 250060H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: bEnd.3-Zellen, Mauslebergewebe, Mauslungengewebe, Maushodengewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IF/ICC: bEnd.3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86229-1-RR bindet in WB, IF/ICC, ELISA JAM-A/CD321 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: JAM-A/CD321 fusion protein Eg3117
• Vollständiger Name: F11 receptor
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: NM_172647.2
• Gene symbol: F11r
• Gene ID (NCBI): 16456
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Junctional Adhesion Molecule-A (JAM-A), also known as CD321 or F11R, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is primarily located in the tight junctions of epithelial and endothelial cells and is also expressed on circulating platelets and leukocytes (PMID: 34533648). Targeting F11R/JAM-A with monoclonal antibodies or peptide antagonists has shown promise in inhibiting tumor growth and metastasis in mouse models (PMID: 37563645).