JMJD6 Polyklonaler Antikörper

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• KD/KO Validated

JMJD6 Polyklonal Antikörper für WB, IHC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16476-1-PBS

Synonyme

Lysyl-hydroxylase JMJD6, KIAA0585, Jumonji domain-containing protein 6, JmjC domain-containing protein 6, EC:1.14.11.-

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  WB analysis of mouse liver using 16476-1-AP (same clone as 16476-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16476-1-AP (JMJD6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 16476-1-AP (same clone as 16476-1-PBS)

  IP result of anti-JMJD6 (IP:16476-1-AP, 4ug; Detection:16476-1-AP 1:500) with K-562 cells lysate 1200ug. This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 16476-1-AP (same clone as 16476-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 16476-1-AP (same clone as 16476-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 16476-1-AP (same clone as 16476-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16476-1-AP (same clone as 16476-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 16476-1-AP (same clone as 16476-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.4 ug JMJD6 Polyclonal antibody (16476-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16476-1-PBS bindet in WB, IHC, FC (Intra), IP, Indirect ELISA JMJD6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JMJD6 fusion protein Ag9590
• Vollständiger Name: jumonji domain containing 6
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC066654
• Gene symbol: JMJD6
• Gene ID (NCBI): 23210
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.