JNK Polyklonaler Antikörper

JNK Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(190)

Kat-Nr. : 24164-1-AP

Synonyme

MAPK8, JNK1A2, JNK1, JNK 2, JNK 1

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  WB analysis using 24164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24164-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 24164-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24164-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 24164-1-AP

  IP result of anti-JNK (IP:24164-1-AP, 4ug; Detection:24164-1-AP 1:10000) with A431 cells lysate 1280 ug.

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  IHC staining of human colon cancer using 24164-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24164-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24164-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 24164-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 24164-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (24164-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 24164-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human JNK (24164-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, Neuro-2a-Zellen, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 185 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

24164-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA JNK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JNK fusion protein Ag21426
• Vollständiger Name: mitogen-activated protein kinase 8
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 42 kDa, 50 kDa
• GenBank-Zugangsnummer: BC130572
• Gene symbol: JNK
• Gene ID (NCBI): 5599
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

• JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

Publikationen

Species	Application	Title
mouse	WB	Nat Metab; Maternal exercise prevents metabolic disorders in offspring mice through SERPINA3C; Authors - Yang Li
	WB	Mol Cell; PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis; Authors - Shouqiao Hou
human	WB	Adv Sci (Weinh); OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression; Authors - Xingran Li
mouse	WB	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
mouse	WB	Clin Transl Med; PINK1 modulates Prdx2 to reduce lipotoxicity-induced apoptosis and attenuate cardiac dysfunction in heart failure mice with a preserved ejection fraction; Authors - Hao Zhang
mouse	IHC	J Nanobiotechnology; Acid-responsive aggregated carrot-derived nanoantioxidants alleviate oxidative stress and restore osteoblast activity; Authors - Jiao Peng