JNK Polyklonaler Antikörper

JNK Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(130)

Kat-Nr. : 51151-1-AP

Synonyme

c Jun N terminal kinase 1, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1

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  WB analysis using 51151-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 51151-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 51151-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 51151-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 51151-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 51151-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 51151-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 51151-1-AP

  Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 51151-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 4T1-Zellen, A431-Zellen, C6-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Neuro-2a-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 124 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

51151-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA JNK und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase 8
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 44-48 kDa, 50-55 kDa
• GenBank-Zugangsnummer: NM_138982
• Gene symbol: JNK
• Gene ID (NCBI): 5599
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

• JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Mlkl knockout mice demonstrate the indispensable role of Mlkl in necroptosis.; Authors - Wu Jianfeng J
mouse	WB	J Crohns Colitis; Mincle/Syk signaling promotes intestinal mucosal inflammation through induction of macrophage pyroptosis in Crohn's disease.; Authors - Wenbin Gong
Mouse	WB	Cell Death Differ; TMCO1 is essential for ovarian follicle development by regulating ER Ca2+store of granulosa cells.; Authors - Zhongshuai Sun
mouse	WB	Br J Pharmacol; Amentoflavone attenuates Listeria monocytogenes pathogenicity through an LLO-dependent mechanism.; Authors - Wang Tingting
mouse	WB	J Transl Med; Angiotensin-(1-7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways; Authors - Xin-Sen Chen
human	WB	Biochim Biophys Acta Mol Basis Dis; Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages; Authors - Ling Zhou