JUN Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

JUN Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

50

Kat-Nr. : 24909-1-AP

Synonyme

Activator protein 1, AP 1, AP1, c Jun, JUN, jun oncogene, P39, Proto oncogene c Jun, Transcription factor AP 1

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Galerie der Validierungsdaten

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  WB analysis using 24909-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24909-1-AP (JUN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 24909-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24909-1-AP (JUN Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 24909-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24909-1-AP (JUN Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 24909-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24909-1-AP (JUN Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 24909-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24909-1-AP (JUN Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of NIH/3T3 using 24909-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using JUN antibody (24909-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: UV treated HeLa cells, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Mit UV behandelte NIH/3T3-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 41 publications below
• IHC: See 3 publications below
• IF: See 11 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

24909-1-AP bindet in WB, IP, IHC, IF, CoIP, ChIP, ELISA JUN und zeigt Reaktivität mit hamster, human, Maus

• Getestete Reaktivität: hamster, human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JUN fusion protein Ag17639
• Vollständiger Name: jun oncogene
• Berechnetes Molekulargewicht: 331 aa, 36 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC068522
• Gene symbol: JUN
• Gene ID (NCBI): 3725
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis[PMID: 22180088]. JUN is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. JUN is a basic leucine zipper (bZIP) transcription factor that acts as homo- or heterodimer, binding to DNA and regulating gene transcription[PMID: 9732876]. In additon, extracellular signals can induce post-translational modifications of JUN, resulting in altered transcriptional activity and target gene expression[PMID:8464713]. More over, it has uncovered multiple layers of a complex regulatory scheme in which JUN is able to crosstalk, amplify and integrate different signals for tissue development and disease. Jun is predominantly nuclear, ubiquitinated Jun colocalizes with lysosomal proteins[PMID: 15469925]. This antibody is a rabbit polyclonal antibody raised against a region of human JUN. Both phosphorylated (p-c-Jun) and unphosphorylated forms of c-Jun, with sizes of 42-45 kDa and 36-39 kDa, respectively are obtain in some experiments. (PMID: 17210646)

Publikationen

Species	Application	Title
mouse	IF	Nature; Mechanisms of stretch-mediated skin expansion at single-cell resolution.; Authors - Mariaceleste Aragona
human	WB	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li ; KD Validated
human	WB,IP	Nat Commun; Proteomic profiling and genome-wide mapping of O-GlcNAc chromatin-associated proteins reveal an O-GlcNAc-regulated genotoxic stress response.; Authors - Yubo Liu
human	WB	Nat Commun; TNF antagonist sensitizes synovial fibroblasts to ferroptotic cell death in collagen-induced arthritis mouse models.; Authors - Jiao Wu
mouse	WB, IF	Biomaterials; Targeted regulation of lymphocytic ER stress response with an overall immunosuppression to alleviate allograft rejection.; Authors - Yingying Shi
rat	WB	Oxid Med Cell Longev; RING Finger Protein 10 Regulates AP-1/Meox2 to Mediate Pirarubicin-Induced Cardiomyocyte Apoptosis; Authors - Hongwei Shi

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