JUN Monoklonaler Antikörper

JUN Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

20

CloneNo.

1G6B11

Kat-Nr. : 66313-1-Ig

Synonyme

Activator protein 1, AP 1, AP1, c Jun, JUN, jun oncogene, P39, Proto oncogene c Jun, Transcription factor AP 1

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Galerie der Validierungsdaten

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  WB analysis of RAW 264.7 using 66313-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66313-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66313-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66313-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66313-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66313-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, A431-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, ROS1728-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 20 publications below

Produktinformation

66313-1-Ig bindet in WB, ELISA JUN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: JUN fusion protein Ag17639
• Vollständiger Name: jun oncogene
• Berechnetes Molekulargewicht: 331 aa, 36 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC068522
• Gene symbol: JUN
• Gene ID (NCBI): 3725
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the molecular weight of JUN?

The molecular weight of JUN is approximately 39 kDa.

What is the cellular localization of JUN?

JUN expression can be located in the nucleus. However, ubiquitinated JUN colocalizes with lysosomal proteins (PMID: 15469925).

 What is the function of JUN?

JUN is a basic leucine zipper (bZIP) transcription factor that recognizes and binds to a consensus heptamer motif of 5'-TGA[CG]TCA-3' in the enhancer region of target genes. JUN acts as either a homo- or heterodimer and binds to the DNA to regulate transcriptional activity, specifically promoting the activity of NR5A1 when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon the stimulation of the cAMP signaling pathway (PMID: 9732876).

 What post-translational modifications is JUN subjected to?

JUN is acetylated at Lys-271 by EP300 (PMID: 11689449). JUN is phosphorylated by PLK3 following exposure to hypoxia or UV irradiation, which leads to increased DNA-binding activity (PMID: 27281822), and also by PAK2 at numerous threonine residues to promote cell proliferation and transformation (PMID: 21177766). JUN is also phosphorylated by DYRK2 at Ser-243, which primes it for subsequent further phosphorylation by GSK3B and reduces its ability to bind DNA (PMID: 22307329).

 What is the role of JUN in disease?

Activation of JUN is linked with proliferation and angiogenesis in invasive breast cancers (PMID: 16733206).

Publikationen

Species	Application	Title
mouse	WB	J Hematol Oncol; PMID: 32552902; Authors - Xinhua Xiao
mouse,human	WB	Cell Biol Toxicol; microRNA-149-5p mediates the PM2.5-induced inflammatory response by targeting TAB2 via MAPK and NF-κB signaling pathways in vivo and in vitro.; Authors - Qiuyue Li
human	WB	Genomics; Integrative epigenomic profiling reveal AP-1 is a key regulator in intrahepatich cholangiocarcinoma.; Authors - Ke He
human	WB	Pharmacol Res; CX-5461 is a potent immunosuppressant which inhibits T cell-mediated alloimmunity via p53-DUSP5.; Authors - Guopin Pan
mouse	WB	J Cell Physiol; Lack of Mof reduces acute liver injury by enhancing transcriptional activation of Igf1.; Authors - Meng Wang
mouse	WB	Front Pharmacol; Azithromycin Attenuates Bleomycin-Induced Pulmonary Fibrosis Partly by Inhibiting the Expression of LOX and LOXL-2.; Authors - Xiang Tong