JUNB Polyklonaler Antikörper

JUNB Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 10486-1-AP

Synonyme

AP 1, jun B proto oncogene, JUNB, Transcription factor jun B

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 10486-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 10486-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10486-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10486-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10486-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10486-1-AP

  IP Result of anti-JUNB (IP:10486-1-AP, 4ug; Detection:10486-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human gliomas using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10486-1-AP (JUNB antibody) at dilution of 1:50 (under 25x lens).

• 

  IHC staining of human testis using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 10486-1-AP

  Immunofluorescent analysis of MCF-7 cells using 10486-1-AP (JUNB antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10486-1-AP

  Immunofluorescent analysis of HeLa cells using 10486-1-AP (JUNB antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, C6-Zellen, HeLa-Zellen, HT-1080.Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Eierstockgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10486-1-AP bindet in WB, IP, IHC, IF, ELISA JUNB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JUNB fusion protein Ag0752
• Vollständiger Name: jun B proto-oncogene
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 42 kDa, 43 kDa
• GenBank-Zugangsnummer: BC004250
• Gene symbol: JUNB
• Gene ID (NCBI): 3726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context

Publikationen

Species	Application	Title
mouse	WB	Signal Transduct Target Ther; MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration; Authors - Jinpeng Du
mouse	WB	J Exp Med; USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.; Authors - Siyuan Chen
mouse,rat	WB	Pharmacol Res; Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.; Authors - Jiale Cai
human	WB	Am J Cancer Res; PMID: 31285951; Authors - Wen-Si Zhao
human	WB	J Proteome Res; Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells.; Authors - Bing Wang
human	WB	Exp Cell Res; An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1; Authors - Xuanxuan Hu