JUNB Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

JUNB Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 10486-1-AP

Synonyme

AP 1, AP-1

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  WB analysis using 10486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10486-1-AP

  IP result of anti-JUNB (IP:10486-1-AP, 4ug; Detection:10486-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human breast cancer using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10486-1-AP (JUNB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10486-1-AP (JUNB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10486-1-AP (JUNB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 10486-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JUNB antibody (10486-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of A431 using 10486-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using JUNB antibody (10486-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HeLa-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Mammakarzinomgewebe, humanes Eierstockgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

10486-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA JUNB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JUNB fusion protein Ag0752
• Vollständiger Name: jun B proto-oncogene
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 38 kDa, 42 kDa
• GenBank-Zugangsnummer: BC004250
• Gene symbol: JUNB
• Gene ID (NCBI): 3726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context

Publikationen

Species	Application	Title
mouse	WB	Signal Transduct Target Ther; MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration; Authors - Jinpeng Du
mouse	WB	J Exp Med; USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.; Authors - Siyuan Chen
rat,mouse	WB	Pharmacol Res; Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.; Authors - Jiale Cai
human	WB	Am J Cancer Res; PMID: 31285951; Authors - Wen-Si Zhao
human	WB	J Proteome Res; Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells.; Authors - Bing Wang
		Oncol Lett; Cigarette smoke extract-induced proliferation of normal human urothelial cells via the MAPK/AP-1 pathway.; Authors - Hao Geng

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