JUNB Rekombinanter Antikörper

JUNB Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

242562G2

Kat-Nr. : CL488-84922-6

Synonyme

242562G2, AP 1, AP-1

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  IF Staining of A431 using CL488-84922-6

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 JUNB antibody (CL488-84922-6, Clone: 242562G2 ) at dilution of 1:200, CL594-Phalloidin (red).

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  IF Staining of HeLa using CL488-84922-6

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 JUNB antibody (CL488-84922-6, Clone: 242562G2 ) at dilution of 1:200, CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, A431-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-84922-6 bindet in IF/ICC JUNB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: JUNB fusion protein Ag0752
• Vollständiger Name: jun B proto-oncogene
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 42 kDa, 43 kDa
• GenBank-Zugangsnummer: BC004250
• Gene symbol: JUNB
• Gene ID (NCBI): 3726
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context