Jagged1 Monoklonaler Antikörper

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• KD/KO Validated

Jagged1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgA

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

CloneNo.

2A7D11

Kat-Nr. : 66890-1-Ig

Synonyme

AGS, AHD, AWS, CD339, HJ1, JAG1, jagged 1 (Alagille syndrome), Jagged1, JAGL1, Protein jagged 1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66890-1-Ig

  WB result of Jagged1 antibody (66890-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Jagged1 transfected HEK-293 cells.

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  WB analysis of HT-29 using 66890-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66890-1-Ig (Jagged1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• FC: See 1 publications below

Produktinformation

66890-1-Ig bindet in WB, FC, ELISA Jagged1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgA
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Jagged1 fusion protein Ag11891
• Vollständiger Name: jagged 1 (Alagille syndrome)
• Berechnetes Molekulargewicht: 1218 aa, 134 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC098393
• Gene symbol: JAG1
• Gene ID (NCBI): 182
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Thiophiler Affinitätschromatograph
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Jagged1 is a type I, membrane- anchored, multidomain protein and a ligand of the Notch receptor. The receptor-ligand interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis. The predicted MW of Jagged1 is 134 kDa, while higher MW around 150-180 kDa had been observed in literations (PMID: 9716576, 9596653).

Publikationen

Species	Application	Title
human	WB	Int J Biol Sci; Linc-KILH potentiates Notch1 signaling through inhibiting KRT19 phosphorylation and promotes the malignancy of hepatocellular carcinoma.; Authors - Xudong Zhang
human	FC	Commun Biol; Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells.; Authors - LinXi Cheng
human	WB	Front Med (Lausanne); IL-7-Treated Periodontal Ligament Cells Regulate Local Immune Homeostasis by Modulating Treg/Th17 Cell Polarization.; Authors - Xin-Yi Yu
human	WB	Cell Biochem Funct; LncRNA TDRG1/miR-214-5p axis affects preeclampsia by modulating trophoblast cells.; Authors - Fengyan Gong
human	WB	Acta Histochem; miR-214-5p suppresses the proliferation, migration and invasion of trophoblast cells in pre-eclampsia by targeting jagged 1 to inhibit notch signaling pathway.; Authors - Fengyan Gong
human	WB	Int J Med Mushrooms; Effects of Tricholoma matsutake (Agaricomycetes) Extracts on Promoting Proliferation of HaCaT Cells and Accelerating Mice Wound Healing; Authors - Wenyu Zhu