• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Jagged1/CD339 Rekombinanter Antikörper

Jagged1/CD339 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241412F1

Kat-Nr. : 84694-1-PBS

Synonyme

JAG1, Jagged1, 241412F1, CD339, HJ1

Filter:
  • Western Blot (WB) analysis of HepG2 cells using Jagged1/CD339 Recombinant monoclonal antibody (84694-1-RR)

    WB analysis of HepG2 using 84694-1-RR (same clone as 84694-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 84694-1-RR (Jagged1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84694-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of IM-9 cells using Jagged1/CD339 Recombinant monoclonal antibody (84694-1-RR)

    IF Staining of IM-9 using 84694-1-RR (same clone as 84694-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed IM-9 cells using Jagged1/CD339 antibody (84694-1-RR, Clone: 241412F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84694-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84694-1-RR

    Affinity and Kinetic Characterization of 84694-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84694-1-RR against Human Jagged1 were performed. The affinity constant is 0.688 nM.

Geprüfte Anwendungen

Produktinformation

84694-1-PBS bindet in WB, IF/ICC, Indirect ELISA Jagged1/CD339 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name jagged 1 (Alagille syndrome)
Berechnetes Molekulargewicht 1218 aa, 134 kDa
Beobachtetes Molekulargewicht150 kDa
GenBank-ZugangsnummerBC098393
Gene symbol Jagged1
Gene ID (NCBI) 182
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Jagged1 is a type I, membrane- anchored, multidomain protein and a ligand of the Notch receptor. The receptor-ligand interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis. The predicted MW of Jagged1 is 134 kDa, while higher MW around 150-180 kDa had been observed in literations (PMID: 9716576, 9596653).