Jagged1/CD339 Rekombinanter Antikörper

Jagged1/CD339 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241412A6

Kat-Nr. : 98243-1-RR

Synonyme

Jagged1, JAG1, Protein jagged-1, HJ1, CD339

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  FC experiment of Transfected HEK-293T using 98243-1-RR

  1x10^6 HEK-293T cells or Jagged1/CD339 transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human Jagged1 Rabbit Recombinant Antibody (98243-1-RR, Clone:241412A6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of Transfected HEK-293T using 98243-1-RR

  1x10^6 HEK-293T cells (left) or Jagged1/CD339 transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Human Jagged1/CD339 Rabbit Recombinant Antibody (98243-1-RR, Clone:241412A6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98243-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98243-1-RR against Human Jagged1/CD339 were performed. The affinity constant is 1.57 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98243-1-RR bindet in FC Jagged1/CD339 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: jagged 1 (Alagille syndrome)
• Berechnetes Molekulargewicht: 1218 aa, 134 kDa
• GenBank-Zugangsnummer: BC098393
• Gene symbol: Jagged1
• Gene ID (NCBI): 182
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Jagged1 (JAG1), also known as CD339, is a type I, membrane-anchored, multi-domain protein and a ligand of the Notch receptor (PMID: 15358557). Jagged1 is composed of a relatively small intracellular domain, a transmembrane domain, and a larger extracellular component containing a cystine-rich region, 16 EGF-like repeats, a DSL domain, and a signal peptide (PMID: 15358557; 26548814). It is widely expressed throughout mammalian development, across many tissues and developmental stages (PMID: 26548814). The Jagged1-NOTCH interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis.