Jagged1/CD339 Rekombinanter Antikörper

Jagged1/CD339 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241412A6

Kat-Nr. : CL647-98243

Synonyme

JAG1, Jagged1, 241412A6, CD339, HJ1

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  FC experiment of Transfected HEK-293T using CL647-98243

  1x10^6 HEK-293T cells or Jagged1/CD339 transfected HEK-293T cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Human Jagged1/CD339 Rabbit RecAb (CL647-98243, Clone:241412A6) or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of Transfected HEK-293T using CL647-98243

  1x10^6 HEK-293T cells or Jagged1/CD339 transfected HEK-293T cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Human Jagged1/CD339 Rabbit RecAb (CL647-98243, Clone:241412A6) (red), or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-98243 bindet in FC Jagged1/CD339 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: jagged 1 (Alagille syndrome)
• Berechnetes Molekulargewicht: 1218 aa, 134 kDa
• GenBank-Zugangsnummer: BC098393
• Gene symbol: Jagged1
• Gene ID (NCBI): 182
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Jagged1 (JAG1), also known as CD339, is a type I, membrane-anchored, multi-domain protein and a ligand of the Notch receptor (PMID: 15358557). Jagged1 is composed of a relatively small intracellular domain, a transmembrane domain, and a larger extracellular component containing a cystine-rich region, 16 EGF-like repeats, a DSL domain, and a signal peptide (PMID: 15358557; 26548814). It is widely expressed throughout mammalian development, across many tissues and developmental stages (PMID: 26548814). The Jagged1-NOTCH interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis.