KALRN Polyklonaler Antikörper

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• KD/KO Validated

KALRN Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19740-1-PBS

Synonyme

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  WB analysis using 19740-1-AP (same clone as 19740-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 19740-1-AP (same clone as 19740-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 19740-1-AP (same clone as 19740-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 19740-1-AP (same clone as 19740-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 19740-1-AP (same clone as 19740-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 19740-1-AP (same clone as 19740-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19740-1-AP (KALRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 19740-1-AP (same clone as 19740-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19740-1-AP (KALRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 19740-1-AP (same clone as 19740-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 19740-1-AP (KALRN antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19740-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA KALRN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: kalirin, RhoGEF kinase
• Berechnetes Molekulargewicht: 340 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: NM_007064
• Gene symbol: KALRN
• Gene ID (NCBI): 8997
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KALRN, also named as DUET, DUO, HAPIP and TRAD, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. KALRN promotes the exchange of GDP by GTP. KALRN activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. KALRN induces lamellipodia independent of its GEF activity. The antibody can recognize isoform1 and isoform2 of KALRN.